Mucin production after infection with C. neoformans.

WT and IFNAR1KO mice were infected intratracheally with C. neoformans. (A) Sections of the lungs on day 14 post-infection were stained with H-E or PAS and observed under a light microscope at ×200. Representative pictures of six mice are shown. Red arrows show the mucin production. (B) The proportion of mucin-producing bronchi (left panel) and the classification of mucin-producing bronchi (right panel) was calculated. Each column represents the mean ± SD of six mice. Experiments were repeated twice with similar results and the representative data are shown. (C) Expression of MUC2, MUC5AC, and MUC5B mRNA in the lungs was measured on day 7 after infection. Each column represents the mean ± SD of five mice. Experiments were repeated twice with similar results and the representative data are shown. (D) Sections of the lungs on day 14 post-infection were immunohistochemical stained with MUC5AC and observed under a light microscope at ×1000. MUC5AC expression is shown in brown. Representative pictures of six mice are shown. NS, not significant; *, p < 0.05.