Mitochondrial localization of <i>S. pombe</i> Coq proteins.

<p>The indicated Coq proteins were fused with GFP and their localization was observed under a fluorescence microscope. The plasmids used in this experiment are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099038#pone.0099038.s002" target="_blank">Figure S2B</a>. (A) A bright-field image and the GFP and MitoTracker Red signals in the <i>Δdlp1</i> strain expressing the Dlp1-GFP fusion protein. The merged GFP and MitoTracker Red signals are also shown. (B–J) GFP signals of the Dps1-GFP fusion expressed in <i>Δdps1</i> cells (B), the Ppt1(Coq2)-GFP fusion expressed in <i>Δppt1</i> cells (C), the Coq3-GFP fusion expressed in <i>Δcoq3</i> cells (D), the Coq4-GFP fusion expressed in <i>Δcoq4</i> cells (E), the Coq5-GFP fusion expressed in <i>Δcoq5</i> cells (F), the Coq6-GFP fusion expressed in <i>Δcoq6</i> cells (G), the Coq7-GFP fusion expressed in <i>Δcoq7</i> cells (H), the Coq8-GFP fusion expressed in <i>Δcoq8</i> cells (I), and the Coq9-GFP fusion expressed in Δ<i>coq9</i> cells (J). All cells were stained with MitoTracker Red to verify the localization of the Coq proteins to mitochondria (data not shown).</p>