Mitochondrial localization of S. pombe Coq proteins.
The indicated Coq proteins were fused with GFP and their localization was observed under a fluorescence microscope. The plasmids used in this experiment are shown in Figure S2B. (A) A bright-field image and the GFP and MitoTracker Red signals in the Δdlp1 strain expressing the Dlp1-GFP fusion protein. The merged GFP and MitoTracker Red signals are also shown. (B–J) GFP signals of the Dps1-GFP fusion expressed in Δdps1 cells (B), the Ppt1(Coq2)-GFP fusion expressed in Δppt1 cells (C), the Coq3-GFP fusion expressed in Δcoq3 cells (D), the Coq4-GFP fusion expressed in Δcoq4 cells (E), the Coq5-GFP fusion expressed in Δcoq5 cells (F), the Coq6-GFP fusion expressed in Δcoq6 cells (G), the Coq7-GFP fusion expressed in Δcoq7 cells (H), the Coq8-GFP fusion expressed in Δcoq8 cells (I), and the Coq9-GFP fusion expressed in Δcoq9 cells (J). All cells were stained with MitoTracker Red to verify the localization of the Coq proteins to mitochondria (data not shown).