Mislocalization of Iav, NompC and Spam in the dTulp mutant.

(A–C) Confocal imaging of the second antennal segment from wild-type, dTulp¹, and genomic rescue flies. The localization of Iav-GFP and NompC are depicted by schematic diagrams. (A) Immunostaining of Iav-GFP (anti-GFP) counterstained with 22C10 which stains neuronal cells except for the outer segments, i.e. cilia. (B) Immunostaining of NompC counterstained with phalloidin that specifically stains actin-rich scolopales. (C) Immunostaining of Spam detected with the monoclonal antibody 21A6.