Migration of mt-PAGFP out of the photoactivation region of muscle fibers.
2013-12-06T03:31:32Z (GMT) by
<p>Representative images of normal (control) (<b>A</b>) and G93A muscle fibers (<b>B</b>). The first image was taken 2 min after the photoactivation because it took a couple of minutes to reconfigure the confocal microscope system to conduct the simultaneous scan. The photoactivation region inside the fiber was marked with the box (white dashed line). Bar: 10 µm. (<b>C</b>) is the time course of mt-PAGFP migration. The linear regression was used to fit the data to evaluate the migration rate. Solid lines are the linear regression of the data after 2 minutes of the photoactivation. By assuming the migration step is zero at time zero, the linear regression was applied to determine the migration rate during the first 2 min (dashed line). The migration rates are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0082112#pone-0082112-t001" target="_blank">Table <b>1</b></a>. (P<0.01).</p>