Mfsd2a KO mice have decreased liver TG with normal liver metabolism.

Oil red-O histology of fasted A, WT and B, KO liver illustrates neutral lipid stores. C, Quantification of TG extracted from fed and fasted WT and KO livers. D, Assay of β-oxidation in liver homogenates from fasted WT and KO littermates (n = 3–4 per genotype, data representative of three independent experiments). E, In vivo uptake of ¹⁴C-oleate in indicated tissues from WT and KO mice, normalized to heart uptake (n = 3 per genotype). F, TLC of lipid extract illustrating in vitro incorporation of ¹⁴C-glycerol and ¹⁴C-oleate by control and Mfsd2a-transfected HEK293 cells (n = 3). CE, cholesterol ester; M/D/TAG, mono-, di-, tri-acylglycerol; PL, phospholipids. G-H, In vivo VLDL production was assayed by measuring G, serum TG and H, cholesterol in fasted WT and KO mice (n>5 per genotype) following treatment with LPL-inhibitor F-127. I, Real-time PCR analysis of PPARα targets and fatty acid synthesis genes from fasted WT and KO liver (n = 3–4). J, Pyruvate tolerance test of 12–14-week old chow-fed WT and KO littermates after an overnight fast (n = 6). Data are displayed as mean ± SEM. * p<0.05, ** p<0.01.