Methylation status of CTCF binding region in Pax6 promoter during ES cell differentiation to radial glial cells.

(A) Detecting changes of DNA methylation in CTCF binding sites during ES cell differentiation and EB formation. (B) Statistical analysis of DNA methylation changes in CTCF binding motifs during ES cell differentiation and EB formation. The genomic DNA harvested from ES cells and EB cells at different stages was treated with sodium bisulfite followed by PCR of the target sequence to generate the template for Ms-SNuPE assays. Methylated cytosines at site 1–3 were revealed by ^[32]P-dCTP incorporation. Symbol “*” indicates the statistical significance determined by Student's t test at p<0.05 (n = 3).