Metformin reduces lipid stores and causes intestinal fluid imbalance.

<p>(<b>A</b>) Quantitation of triacylglycerides (TAGs) in flies treated with 0 mM, 1 mM, 10 mM and 100 mM metformin for 7 days. TAG levels decrease with increasing metformin concentration with significantly lower levels in the 10 mM and 100 mM metformin groups compared to untreated controls (<i>P</i><0.05, n = 10 (2 flies per replicate)). (<b>B</b>) Metformin treatment of female flies does not affect the number of fecal deposits produced per fly over a 24 hour period (<i>P>0.05</i>, Wilcoxen test, n = 5 (5 flies per replicate)). (<b>C</b>) Metformin treatment of female flies does not affect the size of fecal deposits as measured by the mean area of deposits (<i>P>0.05</i>, Wilcoxen test, n = 5 (5 flies per replicate)). (<b>D</b>) Female flies fed with 100 mM metformin produce more concentrated fecal deposits as measured by increased average dye intensity (<i>P</i><0.05, Wilcoxen test, n = 5 (5 flies per replicate)). (<b>E</b>) Female flies fed with 100 mM metformin produce more RODs as a percentage of their total excreta output (<i>P</i><0.05, Wilcoxen test, n = 5 (5 flies per replicate)).</p>