posted on 2014-09-11, 02:46authored byKevin M. Vannella, Luke Barron, Lee A. Borthwick, Kristen N. Kindrachuk, Prakash Babu Narasimhan, Kevin M. Hart, Robert W. Thompson, Sandra White, Allen W. Cheever, Thirumalai R. Ramalingam, Thomas A. Wynn
IL-4Rαflox/ΔLysMCre mice (open bars) and IL-4Rαflox/Δ littermate controls (solid bars) were infected percutaneously with 35 cercariae. From mice infected for 9 weeks, CD45+ SiglecF- CD11b+ Ly6G- F4/80+ CD64+ liver leukocytes were sorted and separated based on Ly6C expression with a flow cytometer. Gene expression was measured by qPCR (n = 3; *p<0.05, ***p<0.001). Fold change is displayed relative to gene expression from CD45+ SiglecF- CD11b+ Ly6G- F4/80+ CD64+ Ly6C+ cells sorted from naïve IL-4Rαflox/Δ littermate control livers. Data shown are mean ±SEM and represent at least two independent experiments.