Macrophage and T cell infiltration in lesions of LDLR<sup>−/−</sup>p110γ<sup>−/−</sup> compared to LDLR<sup>−/−</sup>p110γ<sup>+/−</sup> mice.

<p>Aortic sinus sections were studied in LDLR<sup>−/−</sup>p110γ<sup>+/−</sup> (females, <i>n</i> = 6) and LDLR<sup>−/−</sup>p110γ<sup>−/−</sup> mice (females, <i>n</i> = 7) after two months on a high-fat diet. (<b>A</b>) Representative photomicrographs of Mac-3<sup>+</sup> cells in aortic sinus sections after immunohistochemical staining. Bar = 200 μm. Arrows indicate Mac-3<sup>+</sup> area. (<b>B</b>) Percentage of Mac-3<sup>+</sup>-stained area relative to total lesion area, quantified with ImageJ software. (<b>C</b>) Representative photomicrographs of CD3<sup>+</sup> cells in aortic sinus sections after immunohistochemical staining. Bar = 200 μm. (<b>D</b>) Percentage of CD3<sup>+</sup> cells relative to total lesion area, quantified with ImageJ. (<b>E</b>) Representative photomicrographs of immunofluorescent staining for vascular smooth muscle cells (αSMA<sup>+</sup>) in aortic sinus sections from LDLR<sup>−/−</sup>p110γ<sup>+/−</sup> and LDLR<sup>−/−</sup>p110γ<sup>−/−</sup> mice after two months on a high-fat diet (<i>n</i> = 6 females/genotype). Bar = 100 μm. (<b>F</b>) Percentage of αSMA<sup>+</sup> area relative to total lesion area, quantified with ImageJ. Mean ± SD. Student’s <i>t</i>-test.</p>