Macrophage and T cell infiltration in lesions of LDLR^−/−p110γ^−/− compared to LDLR^−/−p110γ^+/− mice.

Aortic sinus sections were studied in LDLR^−/−p110γ^+/− (females, n = 6) and LDLR^−/−p110γ^−/− mice (females, n = 7) after two months on a high-fat diet. (A) Representative photomicrographs of Mac-3⁺ cells in aortic sinus sections after immunohistochemical staining. Bar = 200 μm. Arrows indicate Mac-3⁺ area. (B) Percentage of Mac-3⁺-stained area relative to total lesion area, quantified with ImageJ software. (C) Representative photomicrographs of CD3⁺ cells in aortic sinus sections after immunohistochemical staining. Bar = 200 μm. (D) Percentage of CD3⁺ cells relative to total lesion area, quantified with ImageJ. (E) Representative photomicrographs of immunofluorescent staining for vascular smooth muscle cells (αSMA⁺) in aortic sinus sections from LDLR^−/−p110γ^+/− and LDLR^−/−p110γ^−/− mice after two months on a high-fat diet (n = 6 females/genotype). Bar = 100 μm. (F) Percentage of αSMA⁺ area relative to total lesion area, quantified with ImageJ. Mean ± SD. Student’s t-test.