Figure_10.tif (319.78 kB)

Macrophage activation by laminarin anchored (laminarin-BAM) or covalently bound (laminarin–SMCC) to tumor cells.

figure

posted on 2014-01-13, 02:57 authored by Tereza Janotová, Marie Jalovecká, Marie Auerová, Ivana Švecová, Pavlína Bruzlová, Veronika Maierová, Zuzana Kumžáková, Štěpánka Čunátová, Zuzana Vlčková, Veronika Caisová, Petra Rozsypalová, Katarína Lukáčová, Nikol Vácová, Markéta Wachtlová, Jiří Salát, Jaroslava Lieskovská, Jan Kopecký, Jan Ženka

B16-F10 cells were cultured with PMJ2R cells for indicated time in the presence of 0.05 mM laminarin-BAM. Free laminarin at the same concentration was used as a control. NF-κB kinase activation was assessed by immunoblotting with antibody specifically recognizing only phosphorylated form. β-actin is shown as a loading control. Two independent experiments were performed. Representative blots are shown (A). In further experiment laminarin-SMCC was covalently bound to B16-F10 cells prior seeding with PMJ2R cells. Intact B16-F10 melanoma cells were used as a control. NF-κB kinase activation was assessed as previously described. Resulting blots are shown (B).