MS-275 enhances expression of acetyl-histone H3 in obstructed kidneys.

Kidney tissue lysates were subject to immunoblot analysis with specific antibodies against acetyl-histone H3 (A). Expression levels of acetyl-histone H3 and histone H3 were quantified by densitometry and normalized with histone H3 (B) and GAPDH, respectively (C). Data are represented as the mean ± SEM (n = 6). Means with different superscript letters are significantly different from one another (P<0.05). Photomicrographs illustrate acetyl-histone H3 with immunofluorescent staining after various treatments as indicated (D).