posted on 2013-12-16, 03:49authored byGozde Korkmaz, Kumsal Ayse Tekirdag, Deniz Gulfem Ozturk, Ali Kosar, Osman Ugur Sezerman, Devrim Gozuacik
Schemes showing ATG4C (A) and (B) BECN1 MRE (top), or their mutated versions (bottom) placed in the 3' UTR of the luciferase gene in the pGL3 vector. Mutated residues were italicized. MIR376A seed target sequence was underlined. (C and D) Normalized luciferase activity measuremets of lysates from 293T cells that were co-transfected with either MIR-CNT or MIR376A plasmids together with the wild-type or mutant luciferase constructs (mean ± S.D. of independent experiments, n = 3 for ATG4C tests, *p<0,05 and n = 5 for BECN1 tests, ***p<0,01).