MC-12 ameliorates colitis induced by both DSS and TNBS.

Paraffin sections of colonic tissues were stained with hematoxylin & eosin and their histological scores were determined as in Methods. A: Normal mucosa of the C57BL/6 mouse. B: Severe inflammation including infiltration by inflammatory cells, edema, loss of crypts and ulcerations are seen in a DSS + vehicle-treated mouse. C, D: MC-12, 5 or 25 mg/kg, IP, significantly decreased DSS-induced colonic inflammation. E: Treatment with MC-12, 25 mg/kg PO, decreased DSS-induced colonic inflammation. F: Treatment with MC-12, 25 mg/kg, IR, markedly reduced DSS-induced colitis. G: Colonic mucosa from a TNBS vehicle-treated mouse, showing severe crypt loss and inflammatory cell infiltration (arrow). Inset: normal mucosa of a healthy SJL/J mouse. H: Treatment with MC-12 25 mg/kg for 2 days decreased the inflammatory cell infiltration and crypt loss (arrow indicates remaining crypts). I, J: The histological score of the various study groups of both DSS- and TNBS-induced colitis. Values are mean ± SEM. H&E staining; magnification 100x.