MCP-1 induces amylin gene expression independent of CCR2.

A. CCR2 mRNA levels in MIN6 cells treated with 11.6 nM MCP-1 for different periods of time, or unstimulated islets was examined by RT-PCR. CCR2 expression in murine microglia cell line N9 was shown as positive control. NC: negative control without cDNA template. B. MIN6 cells were incubated with control medium (CM) or 0.5 µg/ml pertussis toxin (PTX) for 1 h, then stimulated with 11.6 nM MCP-1 for 9 h and examined for amylin expression by real-time PCR. *p<0.05 vs cells cultured with CM. ^#p<0.05 compared with cells treated with MCP-1 alone. Mean±SD of three independent experiments.