Figure_3.tif (8.53 MB)

Loss of differentiated cells following loss of E-cadherin.

figure

posted on 2013-02-20, 21:51 authored by Marlon R. Schneider, Maik Dahlhoff, David Horst, Benjamin Hirschi, Konrad Trülzsch, Josef Müller-Höcker, Roger Vogelmann, Michael Allgäuer, Markus Gerhard, Sylvia Steininger, Eckhard Wolf, Frank T. Kolligs

(A and B) Staining of absorptive enterocytes for villin. Reduced staining intensity and disruption of the epithelial lining (arrowheads) in the small intestine (A) and colon (B) of Cre⁺Cdh1^fl/fl (KO) mice as compared to controls (WT). (C and D) PAS staining revealed a strong reduction of goblet cells (arrowheads) in the colon (C) and small intestine (D) of Cre⁺Cdh1^fl/fl mice four days after start of induction of recombination as compared to control mice. (E and F) Paneth cells were identified by immunostaining for lysozyme (E) and MMP7 (F). After five days of tamoxifen treatment, Paneth cells remained confined to the base of the crypt in control mice, but were distributed throughout the crypt-villus axis in Cre⁺Cdh1^fl/fl mice (arrowheads). Bars in A–F, 100 µm.