Locomotor behavior summary for the <i>pdf<sup>01</sup></i> mutant and RNAi control lines.

2009-12-14T00:57:41Z (GMT) by Orie T. Shafer Paul H. Taghert
<p>Mutants lacking <i>Pdf</i> display several changes in locomotor rhythms as first described by Renn <i>et al.,</i> (1999). <i>Pdf<sup>01</sup></i> mutants display higher levels of arhythmicity under DD, a shortened free-running period (p<0.0001 by t-test), and an advanced evening peak phase compared to wild-type flies (p<0.0001 by t-test). However, there was no significant difference between these genotypes in the power of the locomotor rhythm of rhythmic flies (p = 0.130) under the experimental conditions used here. All control lines displayed high levels of DD rhythmicity. The <i>uas-Dcr2</i> and <i>uas-PDFrnai</i> elements had little if any effects on locomotor rhythms when present as single copies. However the combination of <i>uas-Dcr2</i> and two copies of <i>uas-PDFrnai</i> resulted in an increased period and significantly delayed evening peak. One-way ANOVA of free-running period indicated a significant difference between genotypes, and a Tukey's multiple comparison test revealed a significant difference between <i>w<sup>1118</sup></i> and <i>uas-dcr2</i> (p<0.05) and between <i>uas-Dcr2/y;;uas-PDFrnai</i> and all other genotypes. These control lines also displayed significant differences in the phase of evening activity (p<0.0001 by ANOVA), with Tukey's test revealing significant differences for all pairs of genotype except <i>w<sup>1118</sup></i> versus <i>uas-Dcr2</i>).</p>

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