Localization of αB-crystallin in H<sub>9</sub>C<sub>2</sub> cells before and after heat stress <i>in vitro</i>.

<p>Immunocytochemical staining, scale bar = 10 µm. αB-crystallin (green color, FITC), nuclei (blue color, DAPI) (a) αB-crystallin was localized in the cytoplasm of non-stressed H<sub>9</sub>C<sub>2</sub> cells. In heat-stressed H<sub>9</sub>C<sub>2</sub> cells, the following were observed at the indicated time points: (b) 20 min, weaker positive αB-crystallin signals localized in the cytoplasm, compared with those of control group; (c) 40 min, αB-crystallin signals localized weakly in the cytoplasm; (d) 60 min, αB-crystallin signals were persistently weaker both in the cytoplasm and over the nucleus; (e) 80 min, αB-crystallin still localized over the nucleus and in the cytoplasm at a lower level; (f) 100 min, most strongly positive αB-crystallin signals localized in the cytoplasm as well as around and over the nucleus.</p>