Localization of α-synuclein in the hippocampal CA1 region.

(A, B) Confocal images of hippocampal neurons double immunostained for vGluT-1 and α-synuclein. The region marked by a white square in A is magnified in B. α-Synuclein is colocalized with vGluT-1 in B (arrowheads). (C, D) Confocal images double immunostained for α-synuclein and GAD. The region marked by a white square in C is magnified in D. As indicated by arrows in D, colocalization of α-synuclein and GAD was not detected. Mouse brains from two littermates were used for the immunohistochemical study. This experiment was repeated three times, and the differential expression pattern of α-synuclein was reproducibly confirmed. Panels A and C were used for the quantitative colocalization analyses shown in Fig. S2. Scale bars: 10 µm. indicates stratum pyramidale.