Live cell imaging of cytoplasmic vRNPs along microtubules.

<p>(<b>A</b>) Live cell imaging was carried out using MDCK cells expressing AcGFP-α-tubulin. Pseudo-positive signals (yellow), the microtubule networks (green), and vRNPs (red) were indicated as arrows. (<b>B</b>) Cropped and each color-split image of the indicated area (white box in panel A). Sequential images were shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0021123#pone.0021123.s013" target="_blank">Video S3</a>. (<b>C</b> and <b>D</b>) Time-split images of the merged images and the mAb61A5 channel images in the cropped area, respectively. Elapsed time from the first acquisition was indicated on each image. A vRNP signal (arrowheads in D) moved (event 1, 33.6 to 36.0 s), paused (36.0 to 38.4 s), and moved again (event 2, 38.4 to 40.8 s). Scale bars  = 5 µm.</p>