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Lipofection of HMEC-1 and 2H11cells with siRNA against endoglin reduced tube formation.

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posted on 2013-03-06, 13:43 authored by Tanja Dolinsek, Bostjan Markelc, Gregor Sersa, Andrej Coer, Monika Stimac, Jaka Lavrencak, Andreja Brozic, Simona Kranjc, Maja Cemazar

Original images of tubular complexes of HMEC-1 cells (A)and corresponding binary masks (B) 2 days after the transfectionwith different siRNA molecules: untreated control cells (HMEC-1),cells transfected with Lipofectamine RNAiMAX only (LF), h_siRNA529, h_siRNA 240, h_siRNA 241 and negative control siRNA (siRNACtrl). Original images of tubular complexes of 2H11 cells (C)and corresponding binary masks (D) 2 days after the transfectionwith different siRNA molecules: untreated control cells (2H11),cells transfected with Lipofectamine RNAiMAX only (LF), m_siRNA868, m_siRNA 869, m_siRNA 150 and negative control siRNA (siRNACtrl). Scale bar: 100 µm.

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