Lipid MALDI ion images reveal differences in PC zonation across the liver lobule.

Representative MALDI IMS images of selected liver specimens obtained from obese subjects with normal liver histology (A–D), SS (E–H), and NASH (I–L). (A, E, I) Photomicrographs of H&E stained sections from each specimen (far left column) indicate lobular zones (zone 1 - Z1; zone 3 - Z3). Comparison of selected PCs by disease state: MALDI images of m/z 796.52 PC 34∶2 [M+K]⁺ in obese normal (B), SS (F), and NASH (J) specimens. MALDI images of m/z 820.52 PC 36∶4 [M+K]⁺ in obese normal (C), SS (G), and NASH (K) specimens. MALDI image of m/z 782.54 PC 34∶1 [M+K]⁺ in obese normal (D), SS (H), and NASH (L) specimens. Ion intensity color scale for all ion images is shown at the top of panels B and C. Scale bar = 500 µm.