Linearity of the telomeric repeat amplification protocol (TRAP) using reverse primer-linked probe in tumor tissue.

Serial diluted protein extracts from 2 µg to 0.0002 µg were tested for telomerase activity in one case of squamous cell carcinoma. Results in gel electrophoresis (left), amplification plot (above) and standard curves (below) consistently indicated the linear relationship between the telomerase activity and protein concentration, while 6 represented a negative control.