Lack of p100 cooperates with TNF to increase binding of p52 and RelB to the κB elements in the Enpp2/Atx promoter.

Wild-type and p100^−/− primary MEFs were stimulated with recombinant murine TNF (20 ng/ml) for 6 and 24 h or were left untreated and subsequently nuclear proteins were isolated. In vitro binding of NF-κB subunits p52 (A) and RelB (B) to the κB target sites in the Enpp2/Atx promoter was determined as in Figure 2. Data are presented as mean values ± SD from n = 3 independent experiments. Significant differences (P≤0.05) in p52 and RelB binding were calculated by Student's t-test and are indicated (*).