Ku0063794 and temsirolimus inhibited tumor growth in a xenograft model of RCC.

<p>(<b>A</b>) The treatment with Ku0063794 or temsirolimus significantly inhibited tumor growth in Nu/Nu nude mice. Following subcutaneous injection of 786-O cells, mice (5 mice in each group) were treated from days 33–78. The error bars indicate the standard error of the mean. *p<0.05 when the Ku0063794 or temsirolimus group was compared with control. (<b>B</b>) 90 minutes after drug administration, both Ku0063794 and temsirolimus inhibited the mTORC1 pathway <i>in vivo</i> as indicated by decrease in S6P phosphorylation in the tumor tissues, while only Ku0063794 inhibited the mTORC2 pathway <i>in vivo</i> as indicated by decrease in Akt phosphorylation on Ser473. Each lane represents a tumor from a different mouse.</p>