Knockdown of MBP-1 in HFF altered morphology and exhibited senescence-associated β-galactosidase expression.

Panel A: HFF-MBPsi-4 (panel a) and HFF-control (panel b) were grown to near confluency and stained with H & E. MBP-1 knockdown fibroblasts displayed an irregular, enlarged cellular morphology as compared to the control siRNA transfected cells. Panel B: HFF-MBPsi-4 cells and control-HFF were grown in plates and senescence-associated β-galactosidase assay was performed. MBP-1 knockdown HFF displayed a significantly higher senescence associated β-galactosidase activity (panel a), unlike in control HFF (panel b).