Figure_4.tif (222.97 kB)
Kinetics of uracils excision reported to AP endonuclease activity.
figure
posted on 2013-02-20, 18:58 authored by Emeline Dubois, Dolores Córdoba-Cañero, Sophie Massot, Nicolas Siaud, Bertrand Gakière, Séverine Domenichini, Florence Guérard, Teresa Roldan-Arjona, Marie-Pascale DoutriauxA - Schema showing the in vitro assay that allows to measure Ung activity. B - Protein extracts from Ws plants or RNAi/DUT1-5 plants were used to measure Ung followed by AP endonuclease activity (U∶G) as compared to AP endonuclease activity alone (AP∶G) at different time points. Error bars correspond to two independent experiments, done with two independent protein extracts.
