JNK maintains high levels of GRP78 in human CCA cells.

<p>(A) After treated with SP600125 (SP, 20 µM) for indicated time periods, GRP78 was analyzed using western blot in QBC939, RBE and HCCC-9810 cells. (B) After transfected with siJNK for 60 h, GRP78 was analyzed using western blot in QBC939, RBE and HCCC-9810 cells. (C) After treated with salubrinal (Sal, 25 µM) for 30 h with or without SP600125 (SP, 20 µM) preincubation for 1 h, GRP78 was analyzed using western blot in HepG2 cells. (D) After treated with tunicamycin (Tun, 2.0 µg/ml) for 24 h with or without SP600125 (SP, 20 µM) preincubation for 1 h, GRP78 was analyzed using western blot in HepG2 cells.</p>