JAM-A knockdown inhibited cancer cell proliferation.
(A) MTT assay was performed after JAM-A siRNA treatment. The absorbance at 490 nm at different time points was observed. Data are shown as the mean ± SD of representative experiments, *p<0.05; **p<0.01. 3 independent experiments with 5 internal replicates per experiment were performed with similar results. (B) Assessment of clonogenic potentials of the JAM-A-depleted cancer cells. Complete fields per plate were photographed and presented. Data are shown as representative colony formation assay (Left panel), the number of colonies of triplicate values in a representative experiment was counted, Columns, mean; bars, SD. **p<0.01 (Right panel). 3 independent experiments in triplicate were conducted with similar results.