Iron regulation of transferrin receptor, ferroportin and ferritin is intact in SK-Mel-5 cells.

Treatment of HeLa or SK-Mel-5 cells with 100 µM DFO for 24 hrs resulted in increased expression of the transferrin receptor in both cell lines, and a decrease in ferroportin and ferritin in SK-Mel-5 cells. Baf treatment had a similar effect, with the exception that ferroportin expression in SK-Mel-5 cells was increased by Baf. HeLa cells did not express ferroportin and SK-Mel-5 cells had very low expression of DMT1. TfR, transferrin receptor. DMT1, divalent metal transporter 1. FPN1, ferroportin 1. FTH, ferritin H heavy chain. The immunoblots shown are representative of two independent experiments.