Intra-islet T_reg cells reverse T_eff cell-mediated abrogation of chemokine secretion by ß-islet cells.

β-islet cells were isolated from 4-week-old BDC2.5 mice. (A) Expression of CXCR3 chemokines was compared between insulin⁺ cells and glucagon⁺ cells, and unstained controls. (B), NOD.TCRα^-/- mice received T_reg or T_eff cells either alone or at the indicated T_reg/T_eff cell ratios. When mice receiving T_eff cells alone became hyperglycemic (>33mmol/L), CXCL10 expression (MFI) in β-islet cells was compared between groups. (C) IFN-γR ΔMFI (ΔMFI was calculated by subtracting the isotype control MFI from IFN-γR antibody MFI) and was compared between β (insulin⁺), α (glucagon⁺) and δ (somatostatin⁺) cells isolated from 4-week-old BDC2.5 mice. (5B, 5C n = 5).