Interactions between PARPs and PARGs.

(A) PARP1 associates in vivo with PARP2. (B) PARG1 associates in vivo with PARG2. (C) PARP1 associates in vivo with PARG1 and PARG2. (D) PARP2 associates in vivo with PARG1 and PARG2. The indicated proteins were transiently expressed in N. benthamiana. Input lanes were loaded with total protein extracts, IP lanes were loaded with immunoprecipitation products. Immunoprecipitations were performed with anti-GFP antibodies and immunoblots were analyzed with anti-GFP or anti-myc antibodies, as noted. These experiments were repeated twice with similar results.