Interaction properties of Langerin to different glycosaminoglycans.

<p>(A-C) Structure of glycosaminoglycan disaccharides. Disaccharide units for heparin/HS (A), CS/DS (B) or KS (C). R = COCH<sub>3</sub> or SO<sub>3</sub>H; X = H or SO<sub>3</sub>. The star indicates presence of glucuronic or iduronic acid C-5 epimers. D) SPR inhibition experiment of Langerin ECD/heparin interaction by 15 kDa heparin. Langerin ECD concentration was 100 nM and the heparin concentration range was from 7.8 nM to 2 µM with 2-fold serial dilution factor. E) IC<sub>50</sub> values obtained with heparin, heparin treated with HSulf2 and HS. F) Histogram representation of CS/DS IC<sub>50</sub>. SPR inhibition experiment of Langerin ECD/heparin interaction by 15 kDa heparin. Langerin ECD concentration was fixed at 100 nM and the heparin concentration range was from 7.8 nM to 2 µM with 2-fold serial dilution factor. C) Histogram representation of GAGs IC<sub>50</sub>.</p>