Interaction properties of Langerin to different glycosaminoglycans.

(A-C) Structure of glycosaminoglycan disaccharides. Disaccharide units for heparin/HS (A), CS/DS (B) or KS (C). R = COCH₃ or SO₃H; X = H or SO₃. The star indicates presence of glucuronic or iduronic acid C-5 epimers. D) SPR inhibition experiment of Langerin ECD/heparin interaction by 15 kDa heparin. Langerin ECD concentration was 100 nM and the heparin concentration range was from 7.8 nM to 2 µM with 2-fold serial dilution factor. E) IC₅₀ values obtained with heparin, heparin treated with HSulf2 and HS. F) Histogram representation of CS/DS IC₅₀. SPR inhibition experiment of Langerin ECD/heparin interaction by 15 kDa heparin. Langerin ECD concentration was fixed at 100 nM and the heparin concentration range was from 7.8 nM to 2 µM with 2-fold serial dilution factor. C) Histogram representation of GAGs IC₅₀.