Figure_7.tif (569.99 kB)
Interaction of VBP with AMPPNP (ATP analog) by isothermal titration calorimetry (ITC).
figure
posted on 2014-03-13, 09:02 authored by Abhilash Padavannil, Chacko Jobichen, Yang Qinghua, Jayaraman Seetharaman, Adrian Velazquez-Campoy, Liu Yang, Shen Q. Pan, J. SivaramanRepresentative ITC assays are shown. The upper part of each panel shows the thermogram (thermal power vs. time) after baseline correction and the bottom part of each panel shows the binding isotherm (normalized heat vs. molar ratio of reactants). (A) Calorimetric titration for VBP. (B) Calorimetric titration for VBP N186D. (C) Calorimetric titration for HEPN.
