Inhibition of NA-induced Elk1 activation by silodosin.

Prostate tissue from each patient was allocated to three samples, which were stimulated with NA (30 µM) for 15 min, or remained unstimulated. Silodosin (3 µM) or solvent (3 µl DMSO) were added 15 min before NA as indicated. All samples were exposed for identical total periods to experimental conditions to prevent agonist-unspecific effects. In each experiment, Elk1 in NA-stimulated samples without silodosin ( =  DMSO) was set to 100%, and values for samples with silodosin were referred to that sample. (A) Inhibition of Elk1 activity by silodosin in NA-stimulated prostate samples (n = 6 patients), detected by EMSA. (B) Inhibition of Elk1 phosphorylation by silodosin in NA-stimulated prostate samples (n = 6 patients), assessed by Western blot analysis. Shown are representative experiments, and densitometric quantification of all experiments (means±SEM).