Inhibition of H9N2 virus infection in MDCK cells.

(a) HA RNA quantification in supernatant. RNA was quantified with a qPCR assay at 12, 24, 36, and 48 h, respectively. The concentrations of HA-RNA in a logarithmic transformation were determined. (b) HA RNA quantification in cells. The cells harvested at 4, 8, and 12 h were quantified in a qPCR assay. The ratio of viral load to cell number, with a logarithmic transformation, was measured. Results are the mean ± SD values obtained from three independent experiments. The c-A9 represents A9 incubated with its complementary sequence, and the c-B4 represents B4 incubated with its complementary sequence. **, difference between the aptamer groups (A9 or B4) and the no aptamer groups, P < 0.05. *, significant difference between the aptamer groups (A9 or B4) and the no aptamer groups, P < 0.01. ConS, Control Sequence (5’-AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3’).