Infection with <i>T. rangeli</i> reduces the NOS activity and the levels of NOS protein in the salivary glands of <i>R. prolixus</i>.

<p>A. <i>Rhodnius</i> were dissected 7 days after control injection of water or <i>T. rangeli</i> and assayed for NADPH-diaphorase activity. Results from three experiments were evaluated statistically using the Student t test (* p<0.05). B. Salivary gland extracts from control or <i>T. rangeli-</i>injected insects were fractionated by SDS-PAGE and transferred to a nitrocellulose membrane. The membranes were incubated with primary antibody anti-NOS and then with an anti-rabbit antibody conjugated to alkaline phosphatase. This experiment was performed three times. Tr, <i>Trypanosoma rangeli</i> cells evalutated for NOS blotting. N, salivary glands from non-injected insects. C, control salivary glands from insects injected with water. I, Salivary glands from <i>T. rangeli</i>-injected insects. C. NADPH-diaphorase activity was measured in salivary gland extracts of salivary glands three days after injection with 100 ng of glycolipids from either <i>T. rangeli</i> (Tr GIPL), <i>P. serpens (</i>Ps GIPL<i>)</i> or <i>T. cruzi</i> eGPI-mucin (Tc Mucin). The experiment was performed three times and analyzed by ANOVA (* p<0.05).</p>