Individual assembly of full-length mRNA sequences.

(a) Length distribution of synthetic long reads (minimum length 500 bp) from HCT116 mRNA. (b) Length distribution of synthetic long reads (minimum length 500 bp) from HepG2 mRNA. (c) Box plots showing the number of splice junctions spanned by short reads and synthetic long reads. The axis is broken between 5–10 junctions spanned and the scale changed; a version with a standard axis is presented as S12 Fig. Inset: 97% of the junctions identified in the synthetic reads are known, providing validation for the method.