Indirect immunofluorescence microscopy in HL60 and U937 cells treated with T44Bf.

Synchronized cells were treated with T44Bf (10 μM and 20 μM) or Vincristine (200 nM) for 15h. Cells were fixed and stained to detect α-tubulin (red) and counterstained by Hoechst for DNA (blue). Mounted slides were visualized under 1000X magnification on a Nikon Eclipse E200 fluorescence microscope. Yellow arrows indicate extra spindle poles and green arrows indicate unaligned chromosomes. Images are representative of three independent experiments.