Increased mRNA levels of pro-inflammatory genes and decreased levels of anti-inflammatory genes in Sirt2^−/− mouse-derived BMDMs.

(A) Sirt2 mRNA levels in Sirt2^+/+ (+/+) and Sirt2^−/− (−/−) mice. (B and C) mRNA levels of pro-inflammatory cytokine genes (Il1β, Tnfα, Il6, Mcp1) (B) and anti-inflammatory genes (Il4r, Il10) (C) in BMDMs from Sirt2^+/+ and Sirt2^−/− mice under basal conditions. n = 3/group. (D) mRNA levels of pro-inflammatory genes in BMDMs from Sirt2^+/+ and Sirt2^−/− mice upon LPS treatment. (E) mRNA levels of Gata3, Arg1, and Cd11c in BMDMs from Sirt2^+/+ and Sirt2^−/− mice upon IL4 treatment. Results are expressed as the mean ± SEM. *P<0.05; **P<0.01; ***P<0.001.