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Increased Sirt1 protein levels in cells incubated in CR sera.

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posted on 21.02.2013 by Joanne S. Allard, Leonie K. Heilbronn, Carolina Smith, Nicole D. Hunt, Donald K. Ingram, Eric Ravussin, Rafael de Cabo

Sirt1 expression was evaluated using western blotting techniques. (A) Shows representative blots of Sirt1 and β-actin protein. The first and second bands of each pair of bands represent relative levels of Sirt1 protein induced in HepG2 cells by baseline (BL) and 3 month (3 M) sera samples, respectively. (B) Shows changes in sirt1 levels relative to baseline. For C, CR and CREX n = 9, 11 and 11 respectively. * p = 0.018.

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