<i>In vivo</i> effect of miR-21 on CNE1 cell growth in nude mice.

2014-11-03T03:52:19Z (GMT) by Hesheng Ou Yumei Li Min Kang
<p>CNE1 cells (10<sup>7</sup> cells/tumor), treated with miR-21 or control (100 nM for 48 h) without transfection reagents, were injected subcutaneously into the right axilla of nude mice. <b>(A)</b> Growth curves for CNE1 cells treated with miR-21 (n = 8) and control (n = 8) obtained by <i>in vivo</i> proliferation assay. P<0.05 for all. <b>(B)</b> Photographs of tumors from miR-21 and control groups. <b>(C)</b> Photographs of mice from miR-21 and control groups. <b>(D)</b> Representative photomicrographs of CNE1 cells and apoptosis in miR-21 and control groups detected by TUNEL assay. <b>(E)</b> Representative photomicrographs of immunohistochemical staining and the relative levels of Bcl-2 and Bax on xenograft tumor sections obtained from mice in miR-21 and control groups. The cytoplasm was counter-stained with hematoxylin (×400).</p>