In vitro growth response to modulation of AR expression in E006AA cells.

(A) Cell growth analyses of E006AA cells transduced with or without Lentiviral wild-type AR, which were then treated with 10 µM Casodex or an increasing dose of R1881 (1.0 nM to 100 nM). Cell growth is graphed relative to the respective untreated control cells. (B) Comparison of vector only Lentiviral-non-silencing, Lenti-AR, and Lenti-AR-shRNA transduced E006AA cell growth relative to control cells. (C) Colony forming assay comparing parental control cells to non-silencing, and two different anti-AR Lenti-shRNA sequences (3715 and 3718).