<i>In vitro</i> cell assays for functional validation of differentially-expressed protein candidates in HASMCs.
2015-08-25T03:04:18Z (GMT) by
<p>HASMCs were transfected with the specific siRNA mixes listed in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133845#pone.0133845.s006" target="_blank">S1 Table</a> for 18 hr and stimulated with PDGF-BB (25 ng/ml) or TNF-α (10 ng/ml). Each experiment was performed in triplicate and the data in the graph are means ± S.D. of fold changes from three independent experiments (*<i>P</i><0.02, **<i>P</i><0.002). UT, untreated; C, control siRNA-transfected cells; S, cytosolic S-100; N, nuclei; M, membrane. The number designated each bar corresponds to the spot number.</p>