In vitro binding affinity of the parental mAb αD11 towards hNGF and mNGF.

(a) Sequence alignment of mNGF, hNGF and rat NGF (Loop I and Loop II are colored in magenta and green, respectively). (b) Overall structure of NGF and structural comparisons [30], [31] of Loop I (in magenta) and Loop II (in green) from mNGF (PDB_ID: 1BTG, cyan) [28] and hNGF (PDB_ID: 1WWW, green) [29]. This Figure was prepared with PyMOL [32]. (c) ELISA assay with mNGF and hNGF coating (5 µg/ml) and serial dilutions of parental mAb αD11. The experiments were done in duplicate.