<i>In vitro</i> binding affinity of the parental mAb αD11 towards hNGF and mNGF.

<p>(a) Sequence alignment of mNGF, hNGF and rat NGF (Loop I and Loop II are colored in magenta and green, respectively). (b) Overall structure of NGF and structural comparisons <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032212#pone.0032212-Prli1" target="_blank">[30]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032212#pone.0032212-Ye1" target="_blank">[31]</a> of Loop I (in magenta) and Loop II (in green) from mNGF (PDB_ID: 1BTG, cyan) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032212#pone.0032212-Holland1" target="_blank">[28]</a> and hNGF (PDB_ID: 1WWW, green) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032212#pone.0032212-Wiesmann1" target="_blank">[29]</a>. This Figure was prepared with PyMOL <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032212#pone.0032212-DeLano1" target="_blank">[32]</a>. (c) ELISA assay with mNGF and hNGF coating (5 µg/ml) and serial dilutions of parental mAb αD11. The experiments were done in duplicate.</p>