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In vitro amastigote growth assays using tdTomato parasites.
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posted on 2013-02-21, 00:37 authored by Adriana M. C. Canavaci, Juan M. Bustamante, Angel M. Padilla, Cecilia M. Perez Brandan, Laura J. Simpson, Dan Xu, Courtney L. Boehlke, Rick L. Tarleton(A) Amastigotes growth in Vero cells grown in 96 well plates over time in the presence of benznidazole (n = 8). (B) Comparison of IC50 calculations in response to EXO2-04 in 96 and 384 well plates at 3 days of treatment (n = 4). (C) Amastigote growth assay in 96 or 384 well plates using the Colombiana and TCC strain of T. cruzi expressing tdTomato fluorescent protein at 3 days of treatment (n = 8).
