Impact of pair-rule gene orthologue knockdown on pair-rule expression.

All embryos are oriented with anterior left and dorsal side up. Scale bars are 200 µm. Expression of Am-eve (A), Am-run (B) and Am-h (C) Am-run at stage 5–6 appears as six or seven broad stripes across the anterior-posterior axis of the embryo. These stripes subsequently split as development proceeds. Am-eve^RNAi stage 5 embryo stained for Am-run (D) showing loss of all but an abdominal stripe, broader than in controls. Am-h (E) stained Am-eve^RNAi stage 5 embryo showing loss of all segmental stripes and a posterior terminal cap of RNA expression. (F) Am-eve expression in an Am-run^RNAi embryo showing expression only inn the post posterior abdominal stripes. (G). Am-h stripes are disorganized in Am-run^RNAi embryos, forming a broad anterior domain, followed by two sets of a narrow stripe, then a broad one along the length of the embryo. Dark spots (asterisks) are damage to the other side of the embryo (H) Am-h^RNAi embryo stained for Am-eve RNA showing loss and disruption of central stripes, but an anterior stripe and posterior stripes of cells expression of Am-eve still remain. (I) Am-h^RNAi knockdown embryo stained for Am-run RNA showing over-expression of Am-run RNA throughout the embryo except in the anterior terminus. Fluctuations in the intensity of staining indicate some segmental modulation of RNA expression. (J). Am-ftz^RNAi embryo stained for Am-eve RNA showing disruption of anterior stripes of Am-eve leading to fusion of the first 4 stripes of Am-eve expression and three posterior stripes of cells expressing Am-eve. (K) Am-run stained Am-ftz^RNAi embryo showing loss of anterior stripes of expression, and disorganized posterior ones. (L) Am-h stained Am-ftz^RNAi embryo with disorganized stripes and increased background staining.