Immunohistochemical detection of FGFR-2-IIIb in thyroid tissue.

Dewaxed sections of normal thyroid tissue (A, A’), hyperplastic thyroid tissue (B, B’), follicular adenoma (C, C’) and papillary carcinoma (D, D’) were subjected to immunohistochemistry using a home-made antibody directed against FGFR-2-IIIb. (A–D), original magnification 10×, scale bar 20 µm. A significant area in each panel is indicated by a square and an enlargement of this area is shown in the respective (A’–D’) panels (original magnification 20×, scale bar 20 µm). (A–A’) Normal thyroid sections showing positive staining pattern for FGFR-2-IIIb in both cytoplasm (arrow) and cell membrane compartment (arrowhead) of follicular cells. (B–B’) Hyperplastic thyroid sections showing positive staining pattern for FGFR-2-IIIb in follicular cells cytoplasm (arrows). (C–C’), (D–D’) Follicular adenoma and papillary carcinoma samples showing no FGFR-2-IIIb signal in follicular cells (arrows).