Immunohistochemical analysis showing beta-amyloid (Aβ) content in hippocampal tissue after its administration at different concentrations.

Coronal section indicating the location of the probe (hippocampus, black rectangle) counterstained with Mayer Hematoxylin and relative fluorescence micrographs of the area within the black rectangle showing the presence of human Aβ protein. Aβ immunoreactivity (red-PE staining, white arrows) immediately after perfusion of 10 µM, 1 µM and 100 nM Aβ1–40. Nuclear DNA was counterstained with Hoechst 33342 (blue staining). Scale bars for Mayer hematoxylin sections: 200 µm. Scale bars for fluorescent micrographs: 125 µm.