Immunofluorescent localization of tubulin and KIFC1 during the early stage of E. modestus spermiogenesis.
(A) DAPI nuclear staining. (B) Tubulin staining. Tubulin (green staining; arrows) localizes in the cytoplasm and near the nuclear membrane. (C) KIFC1 staining. KIFC1 (red staining; arrows) also localizes in the cytoplasm and near the nuclear membrane. (D). Phase contrast microscopy. This figure shows the general morphological appearance of the spermatid during the early stage of spermiogenesis. (E) Merged immunofluorescent images. Tubulin (green staining; arrows) and KIFC1 (red staining; arrows) co-localize in the cytoplasm and near the nuclear membrane. Nucleus (blue staining). (F) Diagram. This diagram shows the distribution of tubulin (green dots) and KIFC1 (red dots) in the spermatid during the early stage of spermiogenesis. N: nucleus.