Immunofluorescence assay of Vero cells incubated with Stx2.

<p>Cells were incubated with purified Stx2 (100 CD50) or transfected with the pStx2 plasmid and stained with propidium iodide. Presence of Stx2 was detected with polyclonal anti-Stx2 serum and FITC-conjugated goat anti-mouse antibody, and analyzed by confocal microscopy. Original magnification 600X. <b>A:</b> Non-treated Vero cells. <b>B:</b> Cells transfected with the pGEM-T vector alone. <b>C:</b> Cells incubated with purified Stx2. <b>D:</b> Digital zoom (3.5X) of picture C. <b>E:</b> Cells transfected with pStx2. <b>F:</b> Digital zoom (3.5X) of picture <b>E</b>.</p>