Figure_5.tif (4.71 MB)
Immunofluorescence assay of Vero cells incubated with Stx2.
figure
posted on 2013-02-23, 11:28 authored by Leticia V. Bentancor, Marcos F. Bilen, María P. Mejías, Romina J. Fernández-Brando, Cecilia A. Panek, Maria V. Ramos, Gabriela C. Fernández, Martín Isturiz, Pablo D. Ghiringhelli, Marina S. PalermoCells were incubated with purified Stx2 (100 CD50) or transfected with the pStx2 plasmid and stained with propidium iodide. Presence of Stx2 was detected with polyclonal anti-Stx2 serum and FITC-conjugated goat anti-mouse antibody, and analyzed by confocal microscopy. Original magnification 600X. A: Non-treated Vero cells. B: Cells transfected with the pGEM-T vector alone. C: Cells incubated with purified Stx2. D: Digital zoom (3.5X) of picture C. E: Cells transfected with pStx2. F: Digital zoom (3.5X) of picture E.
